Assignment of the gene coding for human cytochromecoxidase subunit VIb to chromosome 19, band q13.1, by fluorescence in situ hybridisation

✍ Scribed by Jan-Willem Taanman; Anneke Y. Veen; Cobi Schrage; Hans Vries; Charles H. C. M. Buys

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 762 KB
• Volume: 87
• Category: Article
• ISSN: 0340-6717
• DOI: 10.1007/bf00200913

✦ Synopsis

A cloned, 40 kb, genomic DNA fragment, containing the last exon of the gene for human cytochrome c oxidase subunit VIb and its flanking sequences, was used as a probe to localize the subunit VIb gene on human metaphase chromosomes. The probe was labelled with Bio-11-dUTP and detected by fluorescence. Subsequent R-banding indicated that the cytochrome c oxidase subunit VIb gene is localized in band 19q13.1, extending the evidence that the human nuclear genes of cytochrome c oxidase are not clustered.