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I-SceI meganuclease mediates highly efficient transgenesis in fish

✍ Scribed by Violette Thermes; Clemens Grabher; Filomena Ristoratore; Franck Bourrat; André Choulika; Jochen Wittbrodt; Jean-Stéphane Joly

Book ID
118602537
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
198 KB
Volume
118
Category
Article
ISSN
0925-4773

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✍ Fong Cheng Pan; Yonglong Chen; Jana Loeber; Kristine Henningfeld; Tomas Pieler 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 743 KB

## Abstract Several experimental approaches have been described to generate transgenic frogs. Here, we report on the application of a novel method in __Xenopus__, making use of __I‐SceI__ meganuclease. The characteristic feature of this endonuclease is that it has an extended recognition site of 18