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Highly efficient transgenesis in Xenopus tropicalis using I-SceI meganuclease

✍ Scribed by Hajime Ogino; William B. McConnell; Robert M. Grainger


Book ID
119327410
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
430 KB
Volume
123
Category
Article
ISSN
0925-4773

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## Abstract Several experimental approaches have been described to generate transgenic frogs. Here, we report on the application of a novel method in __Xenopus__, making use of __I‐SceI__ meganuclease. The characteristic feature of this endonuclease is that it has an extended recognition site of 18