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High-throughput transgenesis in Xenopus using I-SceI meganuclease

✍ Scribed by Ogino, Hajime; McConnell, William B; Grainger, Robert M


Book ID
109945844
Publisher
Nature Publishing Group
Year
2006
Tongue
English
Weight
256 KB
Volume
1
Category
Article
ISSN
1750-2799

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## Abstract Several experimental approaches have been described to generate transgenic frogs. Here, we report on the application of a novel method in __Xenopus__, making use of __I‐SceI__ meganuclease. The characteristic feature of this endonuclease is that it has an extended recognition site of 18