F-prime heterogenotes of dam-3 bacteria segregate F-prime homogenotes at a frequency 30-200 times higher than the isogenic dam+ strain. A hyperrecombination mutant which shows increased recombination between chromosomal duplications was characterized as a dam mutant. The dam-3 allele causes a reduct
Hyper-recombination in uvrD mutants of Escherichia coli K-12
โ Scribed by Arthur, Helen M. ;Lloyd, Robert G.
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 571 KB
- Volume
- 180
- Category
- Article
- ISSN
- 0026-8925
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Mutation of the uvrD gene of Escherichia coli is associated with an increased capacity for genetic recombination. The hyper-recombination effect is abolished by an additional mutation in lexA that limits synthesis of RecA protein and other gene products regulated by LexA repressor, and is not restor
Genetic recombination in Escherichia coli was investigated by measuring the effect of mutations in ruv and rec genes on F-prime transfer and mobilization of nonconjugative plasmids. Mutation of ruv was found to reduce the recovery of F-prime transconjugants in crosses with recB recC sbcA strains by