Evidence of abortive recombination in ruv mutants of Escherichia coli K12

✍ Scribed by Benson, Fiona ;Collier, Simon ;Lloyd, Robert G.

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 888 KB
• Volume: 225
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00269858

✦ Synopsis

Genetic recombination in Escherichia coli was investigated by measuring the effect of mutations in ruv and rec genes on F-prime transfer and mobilization of nonconjugative plasmids. Mutation of ruv was found to reduce the recovery of F-prime transconjugants in crosses with recB recC sbcA strains by about 30-fold and with recB recC sbcB sbcC strains by more than 300-fold. Conjugative plasmids lacking any significant homology with the chromosome were transferred normally to these ruv mutants. Mobilization of the plasmid cloning vectors pHSG415, pBR322, pACYC184 and pUC18 were reduced by 20- to 100-fold in crosses with ruv rec+ sbc+ strains, depending on the plasmid used. Recombinant plasmids carrying ruv+ were transferred efficiently. With both F-prime transfer and F-prime cointegrate mobilization, the effect of ruv was suppressed by inactivating recA. It is proposed that the failure to recover transconjugants in ruv recA+ strains is due to abortive recombination and that the ruv genes define activities which function late in recombination to help convert recombination intermediates into viable products.