This paper describes a method of screening mutagenised populations of an E. coli galA/galB F- prime merodiploid for mutants defective in recombination. The method relies on scoring colonies on Eosin-Methylene Blue agar that have fewer than normal numbers of Gal+ papillae. With a suitable choice of g
Chromosomal transfer of F-merodiploids of recombination-defective mutants of Escherichia coli K-12
β Scribed by S. N. Rudneva
- Publisher
- Springer US
- Year
- 1970
- Tongue
- English
- Weight
- 198 KB
- Volume
- 70
- Category
- Article
- ISSN
- 0007-4888
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Genetic recombination in Escherichia coli was investigated by measuring the effect of mutations in ruv and rec genes on F-prime transfer and mobilization of nonconjugative plasmids. Mutation of ruv was found to reduce the recovery of F-prime transconjugants in crosses with recB recC sbcA strains by