๐”– Bobbio Scriptorium
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Hydroxylation of salicylate as an assay for hydroxyl radicals: A cautionary note

โœ Scribed by Barry Halliwell; Harparkash Kaur; Magnus Ingelman-Sundberg

Book ID
113385523
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
201 KB
Volume
10
Category
Article
ISSN
0891-5849

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Detection of Hydroxyl Radicals by d-Phen
Detection of Hydroxyl Radicals by d-Phenylalanine Hydroxylation: A Specific Assay for Hydroxyl Radical Generation in Biological Systems
โœ Roberto Biondi; Yong Xia; Ruggero Rossi; Nazareno Paolocci; Giuseppe Ambrosio; J ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 177 KB

Hydroxylation of l-phenylalanine (Phe) by hydroxyl radical (\*OH) yields 4-, 3-, and 2-hydroxyl-Phe (para-, meta-, and ortho-tyrosine, respectively). Phe derivative measurements have been employed to detect \*OH formation in cells and tissues, however, the specificity of this assay is limited since