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Hydrogen peroxide amperometric biosensor based on a peroxidase-graphite-epoxy biocomposite

✍ Scribed by A. Morales; F. Céspedes; J. Mũnoz; E. Martínez-Fábregas; S. Alegret

Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
654 KB
Volume
332
Category
Article
ISSN
0003-2670

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✦ Synopsis

An amperometric biosensor sensitive to hydrogen peroxide has been built, using a HRP-graphite-epoxy biocomposite. The proximity of the redox centers of the enzyme and the conductive sites at the surface of the electrode permits a direct regeneration of the enzyme. The response characteristics of this biosensor have been improved by adding powdered platinum to the matrix of the biocomposite. When Pt is absent, the working potential is -300 mV vs. Ag/AgCl and the response is linear between 0.03 rnM and 7 mM. When 3% wt platinum is added to the biocomposite, a stable response is produced at -50 mV vs. Ag/AgCl and the linearity range lies between 0.09 mM and 9 mM. The response time is approximately 2 s for both the cases.

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