Amperometric Biosensor for Hydrogen Peroxide Based on Sol-Gel/Hydrogel Composite Thin Film

✍ Scribed by Xu Chen; Bingquan Wang; Shaojun Dong

• Publisher: John Wiley and Sons
• Year: 2001
• Tongue: English
• Weight: 65 KB
• Volume: 13
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/1521-4109(200110)13:14<1149::aid-elan1149>3.0.co;2-l

✦ Synopsis

A reagentless amperometric hydrogen peroxide biosensor was developed. Horseradish peroxidase (HRP) was immobilized in a novel sol-gel organic-inorganic hybrid matrix that is composed of silica sol and a grafting copolymer of poly(vinyl alcohol) with 4-vinylpyridine (PVA-g-PVP). Tetrathiafulvalene (TTF) was employed as a mediator and could lower the operating potential to À50 mV (versus Ag=AgCl). The sensor achieved 95 % of the steady-state current in 15 s. Linear calibration for hydrogen peroxide was up to 1.3 mM with the detection limit of 2.5610 À7 M. The enzyme electrode retained about 94 % of its initial activity after 30 days of storage in a dry state at 4 C.