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Amperometric glucose biosensor based on an electrocatalytically bulk-modified epoxy-graphite biocomposite

✍ Scribed by F. Céspedes; E. Martínez-Fàbregas; S. Alegret


Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
513 KB
Volume
284
Category
Article
ISSN
0003-2670

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✦ Synopsis


An inexpensive, robust, polishable and easily machinable amperometric glucose transducer was constructed. The biocomposite materials used were graphite, palladium-gold, nonconducting epoxy resin and glucose oxidase. The enzyme retains its bioactivity in the rigid epoxy-graphite matrix. The electrocatalytic oxidation of hydrogen peroxide is enhanced by gold-palladium and it is used as the analytical signal (at 900 mV vs. Ag/AgCl in a pH 7.00 buffered solution with 0.1 M phosphate and 0.1 M KCl). The biosensor exhibits a linear response to glucose in the 0.01-2 mM range and produces steady-state signals within a few seconds (6 s for 95% signal). Simple polishing procedures can be used to generate a fresh bioactive transducer surface. &ywo&s~ Amperometry; Biosensors; Catalytic methods; Biocomposites; Glucose biosensor; Epoxy-graphite biocom-Chemically or physically modified electrodes are very versatile and hold a great promise for electroanalytical applications [1,2]. This is the reason behind the significant research and development efforts seen recently in the field of amperometric devices. The resulting electrodes are increasingly sensitive, selective, stable and inexpensive. They are seen as a viable alternative to bulky and costly analytical equipment, especially in the control and monitoring of biomedical, environmental and industrial processes. In spite of all these advantages, modified amperometric devices have not been widely used as they have not been commercially available. The main reason for this disparity is that modified electrodes are difficult to prepare, especially when activating the surface


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