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Hydrodynamic behavior and molecular conformation of poly(L-lysine HBr) in carbonate buffer solution

✍ Scribed by Kinji Kakiuchi; Hideo Akutsu

Publisher
Wiley (John Wiley & Sons)
Year
1981
Tongue
English
Weight
652 KB
Volume
20
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

In carbonate buffer at pH 10.5, a transparent solution of poly(L‐lysine HBr) was obtained up to fairly high concentration of 3 g/dl at room temperature. The hydrodynamic behavior of the solution has been studied by sedimentation analyses and viscosity measurements. A dimer form for high concentrations and a monomer form for low concentrations were inferred. The dimer and monomer forms were assigned to a β‐structure and α‐helix, respectively, based on the CD and optical rotary dispersion spectra. Using CD spectroscopy, a reversible transition between α‐helix and β‐structure was observed as a function of either poly(L‐lysine HBr) concentration or temperature. An aggregated form which was assigned to the antiparallel pleated sheet appeared at 50°C on the basis of its ir spectrum.

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