𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Human plasma-derived media supplement supports antibody production by hybridoma cells

✍ Scribed by Janet M. Young; Da-Wei Zhang; John Tharakan; Larry Jenkins; Roger Collins; William N. Drohan


Book ID
105181929
Publisher
Springer
Year
1992
Tongue
English
Weight
541 KB
Volume
14
Category
Article
ISSN
1573-0603

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Potassium or sodium phosphate was found to stimulate the production of human monoclonal antibody by human-human hybridoma HB4C5. The addition of 15 mM Na-phosphate (pH 7.4) into serum-free culture medium increased the antibody production up to 4-fold, when seeded at cell density of 1 x 10 5 cells/ml