𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Human papillomavirus type 16 variants isolated from vulvar Bowenoid papulosis

✍ Scribed by Bruno Schwartzman Fang; Antonio Carlos Guedes; Luiz Carlos Muñoz; Luisa Lina Villa

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
554 KB
Volume
41
Category
Article
ISSN
0146-6615

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Tissues from two cases of Bowenoid papulosis of the vulva were found to contain human papillomavirus (HPV) 16 DNA by Southern blot hybridization. Analysis of the hybridization pattern revealed differences in a restriction fragment of one specimen as compared to the HPV 16 DNA prototype. To investigate if these differences could interfere with the expression of such oncogenic viral genomes, the corresponding DNA fragments were cloned and further analyzed. After amplification by PCR and DNA sequencing, a 213 base pairs duplication was mapped in the long control region (LCR) of this HPV 16 variant. One single PCR fragment was obtained from the other Bowenoid papulosis, which is identical in size with the same region in the HPV‐16 prototype. The duplication in the HPV‐16 LCR analyzed in this study maps upstream of a region containing several regulatory elements.

📜 SIMILAR VOLUMES

Detection of human papillomavirus type 1
Detection of human papillomavirus type 16 in bowenoid papulosis and nonbowenoid tissues
✍ Motohiro Endo; Toshiharu Yamashita; Hai-Ying Jin; Yutaka Akutsu; Kowichi Jimbow 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 301 KB

A 58‐year‐old Japanese man visited our clinic in December 2000 with a complaint of an erythematous plaque with reddish papules and pigmentation on the penis shaft and glans. He noticed the lesion 1 month before his visit. He denied any previous homosexual activity. His wife denied any genital skin l

Human papillomavirus type-16-related DNA
Human papillomavirus type-16-related DNA in genital Bowen's disease and in bowenoid papulosis
✍ Hans Ikenberg; Lutz Gissmann; Gerd Gross; Elke-Ingrid Grussendorf-Conen; Harald 📂 Article 📅 1983 🏛 John Wiley and Sons 🌐 French ⚖ 436 KB

## Abstract ^32^P‐labelled DNA of HPV 16 which has been isolated and molecularly cloned from a cervical carcinoma (Dürst __et al.__, 1983) was used to screen the cellular DNAs obtained from 20 different biopsies of Morbus Bowen or Bowenoid papulosis, respectively, by Southern blot analysis. Under c

Human papillomavirus type-16 variants in
Human papillomavirus type-16 variants in Quechua aboriginals from Argentina
✍ María Alejandra Picconi; Lidia Virginia Alonio; Laura Sichero; Viviana Mbayed; L 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 94 KB

## Abstract Cervical carcinoma is the leading cause of cancer death in Quechua indians from Jujuy (northwestern Argentina). To determine the prevalence of HPV‐16 variants, 106 HPV‐16 positive cervical samples were studied, including 33 low‐grade squamous intraepithelial lesions (LSIL), 28 high‐grad

Human papillomavirus type 16 variants in
Human papillomavirus type 16 variants in cervical cancer from an admixtured population in Brazil
✍ Katiana Junes-Gill; Laura Sichero; Paulo Cesar Maciag; Wyller Mello; Vânia Noron 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 91 KB 👁 1 views

## Abstract Several studies indicate that molecular variants of HPV‐16 have different geographic distribution and risk associated with persistent infection and development of high‐grade cervical lesions. In the present study, the frequency of HPV‐16 variants was determined in 81 biopsies from women

Isolation and characterization of a vari
Isolation and characterization of a variant of human papillomavirus type 11 from a nasal inverting (schneiderian) papilloma
✍ Alan Pater; Humphrey Gardner; Don S. Respler; Anthony Jahn; Mary M. Pater 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 524 KB

We have previously reported the presence of a variant of human papillomavirus (HPV) type 1 1 in a nasal inverting papilloma [Respler et al., 19871. In the present study, we have cloned molecularly the DNA of this variant at its unique restriction enzyme Bum HI site into lambda BFlOl phage. Restricti

Sequence variation and physical state of
Sequence variation and physical state of human papillomavirus type 16 cervical cancer isolates from Australia and New Caledonia
✍ Kylie J. Watts; Carol H. Thompson; Yvonne E. Cossart; Barbara R. Rose 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 French ⚖ 356 KB

## Abstract Sequence diversity over 2600 nucleotides of the upstream regulatory region (URR) and the __E6__ and __E2/E4__ genes of 34 human papillomavirus (HPV)16 cervical cancer isolates from Australia and New Caledonia was investigated. One 81 base duplication, 41 single base substitutions and 1