Human nucleolar organizer chromosomes: satellite associations
โ Scribed by T. E. Denton; W. M. Howell; J. V. Barrett
- Publisher
- Springer
- Year
- 1976
- Tongue
- English
- Weight
- 423 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0009-5915
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โฆ Synopsis
The D and G group chromosomes from cultured human lymphocytes exhibit single and multiple satellite associations when stained with silver. Unlike earlier methods this simple and highly repeatable procedure shows physical attachments between satellited regions of various acrocentric autosomes. After studying 1,000 satellite associations from 118 normal individuals, it was found that both single and multiple associations occur with frequencies that correlate with random expectancies.
๐ SIMILAR VOLUMES
Silver-stained cells from 49 parents with a history of several abortions were compared with cells from 35 parents with normal liveborn children. The modal and mean number of silver-stained NORs (Ag-NORs) observed on D- or G-group chromosomes was similar in both groups and between males and females.
A reliable technique for staining human chromosomal nucleolar organizers (NOR's) with silver solutions is described. The NOR's can be selectively stained dark brown by silver solutions leaving the chromosome arms unstained and available for counterstaining with orcein or Giemsa dyes. Unequivocal ide