The derivatization of phenylglyoxylic acid (PGA), a urinary styrene metabolite, gives with diazomethane under ordinary reaction conditions secondary products as expected from the behaviour of other -keto acids Thus reproducible quantitative analysis becomes difficult It is shown that by proper selec
Human exposure to styrene
โ Scribed by M. Guillemin; D. Bauer
- Publisher
- Springer-Verlag
- Year
- 1976
- Tongue
- English
- Weight
- 328 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0340-0131
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โฆ Synopsis
A simple and specific method for the quantitative gaschromatographic (GC) analysis of mandelic and phenylglyoxylic acid (MA and PGA), human urinary styrene metabolites, is described MA is determined by trimethylsilylation and subsequent GC analysis Because of difficulties involved in the direct determination of PGA due to the lack of quantitative and reproducible derivatization methods, this acid is reduced to MA prior to GC analysis By a double determination with and without reduction both acids can be analyzed quantitatively Both analysis show the same accuracy and precision and can therefore be intercorrelated accurately.
๐ SIMILAR VOLUMES
Volunteers (six men and one woman) were exposed by inhalation to styrene within the concentration range of 20 to 200 mg/m 3 The average retention of styrene vapours in the respiratory tract was 71 % The yield of styrene metabolism measured within 24 h was 39 and 17 % for mandelic acid and phenylglyo
Four volunteers were exposed dermally to styrene vapours within the concentration range of 1300 to 3200 mg/m 3 The increase in the levels of mandelic and phenylglyoxylic acids in urine after exposure was strongly noticeable The dermal vapour absorption coefficient (a) was calculated: for styrene it