Human Brain 6-Phosphogluconate Dehydrogenase: Purification and Kinetic Properties
β Scribed by Karen S. Weisz; Philip J. Schofield; Michael R. Edwards
- Book ID
- 111173228
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 785 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0022-3042
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Drosophila melanogaster. The activity level is highest in early third instar larvae and declines to a lower, but relatively constant, level at all later stages of development. The enzyme is localized in the cytosolic portion of the cell. The A-isozymic form of 6-phosphogluconate dehydrogenase was pu
The screening procedure described in the preceding paper allowed a practical purification procedure to be devised that was automated for human 6-phosphogluconate dehydrogenase. The purification needed only two chromatographic steps, first on immobilized Procion Blue HE-GN and then on Phenyl-Sepharos