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HPLC analysis of phenylthiocarbamyl (PTC) amino acids. I. Evaluation and optimization of the procedure

✍ Scribed by P. S. L. Janssen; J. W. van Nispen; P. A. T. A. Melgers; H. W. M. van den Bogaart; R. L. A. E. Hamelinck; B. C. Goverde

Book ID: 112721743
Publisher: Springer
Year: 1986
Tongue: English
Weight: 446 KB
Volume: 22
Category: Article
ISSN: 0009-5893
DOI: 10.1007/bf02268788

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## Abstract For the determination of free amino acids in plasma, the conditions for precolumn derivatization of the amino acids and the chromatographic separation were examined. The isoindole products, formed by the reaction of the primary amino acids with orthophthalaldehyde (OPA), were readily se