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Amino Acid Analysis Protocols Volume 159 || The Analysis of Amino Acids Using Precolumn Derivatization, HPLC, and Electrochemical Detection

✍ Scribed by Cooper, Catherine; Packer, Nicolle; Williams, Keith


Book ID
115471067
Publisher
Humana Press
Year
2000
Weight
132 KB
Category
Article
ISBN-13
9781592590476

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Evaluation of conditions for the HPLC de
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## Abstract For the determination of free amino acids in plasma, the conditions for precolumn derivatization of the amino acids and the chromatographic separation were examined. The isoindole products, formed by the reaction of the primary amino acids with orthophthalaldehyde (OPA), were readily se