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Amino acid analysis by HPLC: Optimized conditions for chromatography of phenylthiocarbamyl derivatives

โœ Scribed by Ray F. Ebert


Book ID
107714254
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
297 KB
Volume
154
Category
Article
ISSN
0003-2697

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โœ David M. Smalley; Peter C. Preusch ๐Ÿ“‚ Article ๐Ÿ“… 1988 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 571 KB

A method is presented for analysis of gamma-carboxyglutamic acid based on its derivatization with phenylisothiocyanate and reverse phase HPLC analysis of the resulting phenylthiocarbamyl derivative. Proteins were hydrolyzed with sodium hydroxide and the hydrolysates were desalted on Dowex 50 eluted