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Homologous transformation of Cephalosporium acremonium with the nitrate reductase-encoding gene (niaD)

✍ Scribed by Whitehead, Michael P.; Gurr, Sarah Jane; Grieve, Carolyn; Unkles, Shiela E.; Spence, David; Ramsden, Martin; Kinghorn, James R.

Book ID
123126486
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
615 KB
Volume
90
Category
Article
ISSN
0378-1119

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The nitrate reductase structural gene (niaD) and an niaD mutant strain were isolated from Aspergillus parasiticus and used to develop a homologous transformation system. A transformation frequency of 110 to 120 transformants per microgram linear DNA was obtained with the 10.9 kb plasmid pSL82, which