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Transformation of the filamentous fungus Gibberella fujikuroi using the Aspergillus niger niaD gene encoding nitrate reductase

✍ Scribed by Sánchez-Fernández, Rocio ;Unkles, Shiela E. ;Campbell, Edward I. ;Macro, Janet A. ;Cerdà-Olmedo, Enrique ;Kinghorn, James R.

Publisher: Springer
Year: 1991
Tongue: English
Weight: 323 KB
Volume: 225
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00269853

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✦ Synopsis

A transformation system for Gibberella fujikuroi based on the Aspergillus niger nitrate reductase gene (niaD) was developed. A strain (designated SG140) carrying a non-reverting niaD mutation (niaD11) was generated by screening mutagenised cells for non-growth on nitrate as sole nitrogen source. Transformation frequencies of 1-2 transformants per microgram DNA were observed when strain SG140 was transformed to nitrate utilisation. Southern blot analyses of niaD+ transformants showed that the vector DNA sequences were integrated into the chromosomal DNA. The results demonstrate that the A. niger niaD gene is expressed in G. fujikuroi.

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