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Transformation of Botrytis cinerea with the nitrate reductase gene (niaD) shows a high frequency of homologous recombination

โœ Scribed by C. Levis; Dominique Fortini; Yves Brygoo


Publisher
Springer-Verlag
Year
1997
Tongue
English
Weight
195 KB
Volume
32
Category
Article
ISSN
0172-8083

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Development of a homologous transformati
โœ Horng, Jason S. ;Chang, Perng-Kuang ;Pestka, James J. ;Linz, John E. ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 363 KB

The nitrate reductase structural gene (niaD) and an niaD mutant strain were isolated from Aspergillus parasiticus and used to develop a homologous transformation system. A transformation frequency of 110 to 120 transformants per microgram linear DNA was obtained with the 10.9 kb plasmid pSL82, which