Development of a homologous transformati
โ
Horng, Jason S. ;Chang, Perng-Kuang ;Pestka, James J. ;Linz, John E.
๐
Article
๐
1990
๐
Springer
๐
English
โ 363 KB
The nitrate reductase structural gene (niaD) and an niaD mutant strain were isolated from Aspergillus parasiticus and used to develop a homologous transformation system. A transformation frequency of 110 to 120 transformants per microgram linear DNA was obtained with the 10.9 kb plasmid pSL82, which