The rate of DNA synthesis in HeLa S3 cells, as measured by incorporation of C14-labeled thymidine, is strongly dependent on protein synthesis at all times during the S phase. The relation between the rate of DNA synthesis and the rate of protein synthesis is linear when measured two or three hours a
Histone protein and DNA synthesis in HeLa cells after thermal shock
β Scribed by Raymond L. Warters; O. Lee Stone
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 868 KB
- Volume
- 118
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
## Abstract Mitotic HeLa cells (M cells) synthesize protein at about 25% of the rate of S phase cells. This decrease in protein synthesis is due to a reduction in the rate of initiation. However, extracts prepared from M cells are almost as active in protein synthesis as S cell extracts. Both cell
## Abstract The protein ERp57/GRP58 is a member of the protein disulfide isomerase family and is also a glucoseβregulated protein, which, together with the other GRPs, is induced by a variety of cellular stress conditions. ERp57/GRP58 is mainly located in the endoplasmic reticulum (ER), but has als
The expression of the 72 kD inducible heat shock protein (hsp72) has been reported to be cell cycle associated in unheated, synchronized HeLa cells. In this study, flow cytometry was used to investigate hsp72 levels through the cell cycle in HeLa cells by dual labeling with propidium iodide and anti
HeLa cells in monolayer cultures were treated with the following inhibitors of DNA synthesis: mitomycin C, nitrogen mustard, fluorodeoxyuridine, hydroxyurea, arabinofuranosylcytosine and high concentrations of thymidine. The concentration of each inhibitor used was, in most cases, just sufficient to
Ischemia of rat liver is followed by recovery or cell death. Since heat shock proteins may be essential to cell survival under stress, we determined levels of heat shock proteins in liver after different periods of blood deprivation and correlated the results with cellular recovery. Cell-free synthe