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Histone-like protein fractions of Kluyveromyces fragilis and their relation to the cell cycle

✍ Scribed by Dr. C. S. Penman; J. H. Duffus

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 715 KB
Volume: 16
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.19760160505

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✦ Synopsis

Abstract

Protein fractions were obtained from Kluyveromyces fragilis by pH titration, a technique used for histone extraction, following the inclusion of a distilled water extraction stage, and the fractions partially characterized. The inclusion of a distilled water step resulted in a tenfold purification of the fraction obtained at pH 2.20 as compared with pH titration alone. In synchronous cultures induced with 2′‐deoxyadenosine or prepared by selection this fraction displayed a stepwise accumulation, and doubled in quantity at a point about one third of a cycle after cell division. The fraction obtained at pH 1.35 resembled calf thymus f2a2 histone in its extraction properties and amino acid composition, and also showed a possible stepwise accumulation. Other fractions appear to accumulate exponentially.

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