Histology of embryogenic responses in soybean anther culture
✍ Scribed by Lia R. Rodrigues; João Marcelo S. Oliveira; Jorge E.A. Mariath; Maria Helena Bodanese-Zanettini
- Publisher
- Springer
- Year
- 2005
- Tongue
- English
- Weight
- 519 KB
- Volume
- 80
- Category
- Article
- ISSN
- 0167-6857
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Anther culture of a three-way cross, (H99 × FR16) × Pa91, resulted in the regeneration of two anther-derived plants which were crossed to produce an F1 progeny. Fourteen S1 families derived from this cross were evaluated for their anther culturability. Dramatic increases in the level of androgenesis
The response of anther culture to culture temperature was studied in detail using many varieties, F1 hybrids and pollen-derived lines of wheat (Triticum aestivum) as materials. The suitable culture temperature for inducing pollen callus (or embryoids) in wheat anther culture ranged from 26 ~ to 30 ~
The numbers of embryogenic (S) grains present in in-situ mature anthers of Nicotiana tabacum L. were compared to the numbers of embryos and plantlets produced in cultured anthers excised at the optimal mitotic stage of development for anther culture. The Feulgen technique of staining embryos caused