Anther culture of maize and the visualization of embryogenic microspores by fluorescent microscopy
β Scribed by G. M. Pace; J. N. Reed; L. C. Ho; J. W. Fahey
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 770 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0040-5752
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The numbers of embryogenic (S) grains present in in-situ mature anthers of Nicotiana tabacum L. were compared to the numbers of embryos and plantlets produced in cultured anthers excised at the optimal mitotic stage of development for anther culture. The Feulgen technique of staining embryos caused
Fertile plants have been obtained from maize (Zea mays L.) embryogenic suspension culture protoplasts. Friable, embryogenic callus initiated from an immature embryo from a cross involving the genotypes A188, B73, and Black Mexican sweetcorn was used to establish a rapidly growing embryogenic suspens
In addition to physical and chemical factors, genotype appears to be a very important factor influencing success in anther culture. Recombination by making crosses with selected responding clones has been introduced as a possible helpful method to positively influence the success and response type v