Crystals of truncated (A425-461) pyridoxal-5'-phosphate (PLP)-dependent mouse ornithine decarboxylase (mOrnDC') have been obtained that diffract to 2.2 resolution (P2,2,2, a = 119.5 A, b = 74.3 A, c = 46.1 A). OrnDC produces putrescine, which is the precursor for the synthesis of polyamines in eukar
Histochemical and cytochemical demonstration of ornithine decarboxylase
β Scribed by R. A. Dodds; J. Chayen
- Publisher
- John Wiley and Sons
- Year
- 1984
- Tongue
- English
- Weight
- 287 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0263-6484
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Trypanosoma brucei ornithine decarboxylase, expressed and purified from E. coli, has been crystallized b y the vapor diffusion method using P E G 3350 as a precipitant. The crystals belong to the monoclinic space group P2, and have cell constants of a = 66.3 A, b = 151.8 A, c = 83.7 A, and p = 101.2
The activites of ornithine decarboxylase (ODC) and ODC inhibitory protein (ODC-antizyme) were studied in Ehrlich ascites tumor cells, separated according to their position in the cell cycle by centrifugal elutriation. Release and/or synthesis of ODC-antizyme was induced by putrescine treatment. Each