Oligoribonucleotides with chain length of 6-9 were synthesized on a polystyrene support using o-nitrobenzyl protection of 2'-hydroxyls via phosphotiriester method. The condensation reactions between N-acvl-5 and nucleoside bound to the resin using l-mesitylenesulfonyl-3-nitro-1H-1,2,4-triazole (MS
✦ LIBER ✦
High-yield synthesis of oligoribonucleotides using o-nitrobenzyl protection of 2′-hydroxyls
✍ Scribed by J.A. Hayes; M.J. Brunden; P.T. Gilham; G.R. Gough
- Book ID
- 108382563
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- French
- Weight
- 282 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0040-4039
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For the efficient synthesis of oligoribonucleotides by the 5'-O-(4,4'-dimethoxytrityl) phosphoramidite approach, the 2'-O-[1-(benzyloxy)ethyl]acetals 56 ± 67 were investigated. Studies with the 2'-O-[1-(benzyloxy)ethyl]-5'-O-(dimethoxytrityl)ribonucleoside 3'-phosphoramidites 56 ± 59 gave, however,