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High-Yield Expression inEscherichia coliand Purification of Mouse Ubiquitin-Activating Enzyme E1

✍ Scribed by Andreia F. Carvalho, Manuel P. Pinto, Cláudia P. Grou, Rui Vitorino, Pedro Domingues, Fumiaki Yamao, Clara Sá-Miranda, Jorge E. Azevedo

Book ID
113093775
Publisher
Humana Press Inc
Year
2011
Tongue
English
Weight
381 KB
Volume
51
Category
Article
ISSN
1073-6085

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The nucleotide sequence of the human cDNA encoding ubiquitin-activating enzyme E1 is more than 99% identical with the human A1S9T cDNA, a gene that has been shown to complement the temperature-sensitive mutant mouse cell line, tsA1S9. The amino acid sequences of the proteins encoded by these two cDN