Mapping and expression of the ubiquitin-activating enzyme E1 (Ube1) gene in the mouse

✍ Scribed by Christine M. Disteche; Eldad Zacksenhaus; David A. Adler; Steven L. Bressler; Bernadette T. Keitz; Verne M. Chapman

• Publisher: Springer-Verlag
• Year: 1992
• Tongue: English
• Weight: 746 KB
• Volume: 3
• Category: Article
• ISSN: 0938-8990
• DOI: 10.1007/bf00352460

✦ Synopsis

The nucleotide sequence of the human cDNA encoding ubiquitin-activating enzyme E1 is more than 99% identical with the human A1S9T cDNA, a gene that has been shown to complement the temperature-sensitive mutant mouse cell line, tsA1S9. The amino acid sequences of the proteins encoded by these two cDNA sequences are identical, and both cDNAs were previously shown to be located in the same region of the human X chromosome; thus, ubiquitin-activating enzyme E 1 and A 1S9T appear to be the same gene, designated UBE1. By in situ hybridization to metaphase chromosomes from male mice and by Southern blot analysis of male and female mouse DNA, we show that, in the mouse, a human UBE1 cDNA probe identified both X-and Y-linked loci.

Ubel is located at band A2 of the mouse X Chromosome (Chr) and Ube2 on the short arm of the Y Chr. This is in contrast to the situation in the human, where there is no evidence for Y-linked sequences related to UBE1. Mapping of the Ubel gene in interspecific backcrosses between Mus spretus and C57BL/6 shows that the Ubel locus maps close to Timp, in a conserved region of the mouse and human X Chrs that include Otc, Cybb, Synl, Timp, and Araf. Expression of Ubel on the inactive X Chr was examined to determine whether this gene is subject to X-Chr inactivation in the mouse, as there is previous evidence that the human UBE1 gene escapes, at least partially, X inactivation. Sequencing of reverse transcriptase polymerase chain reaction (RT-PCR) products from M. spretus, C57BL/6J, and T(X;16)I6H • M. spretus F l female mice indicates that the mouse Ubel gene is subject to X-Chr inactivation in vivo. This represents Offprint requests to: C.M. Disteche a new example of differences between the sex chromosomes of mouse and human.