𝔖 Bobbio Scriptorium
✦   LIBER   ✦

High-Throughput Yeast Two-Hybrid Assays for Large-Scale Protein Interaction Mapping

✍ Scribed by Albertha J.M. Walhout; Marc Vidal

Book ID
115639298
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
134 KB
Volume
24
Category
Article
ISSN
1046-2023

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

High throughput flow cytometry based yea
High throughput flow cytometry based yeast two-hybrid array approach for large-scale analysis of protein-protein interactions
✍ Jun Chen; Mark B. Carter; Bruce S. Edwards; Hong Cai; Larry A. Sklar 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 733 KB

## Abstract The analysis of protein‐protein interactions is a key focus of proteomics efforts. The yeast two‐hybrid system (Y2H) has been the most commonly used method in genome‐wide searches for protein interaction partners. However, the throughput of the current yeast two‐hybrid array approach is

A yEGFP-based reporter system for high-t
A yEGFP-based reporter system for high-throughput yeast two-hybrid assay by flow cytometry
✍ Jun Chen; Jianhong Zhou; Weon Bae; Claire K. Sanders; John P. Nolan; Hong Cai 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 308 KB 👁 1 views

## Abstract The yeast two‐hybrid (Y2H) assay is a widely used method to study protein–protein interactions, a major objective in postgenome research. Despite the tremendous utility of the Y2H assay, several issues including accuracy, speed, automation, and cost‐effectiveness limit its application i