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High-throughput mammalian two-hybrid screening for protein-protein interactions using transfected cell arrays

โœ Scribed by Andrea Fiebitz; Lajos Nyarsik; Bernard Haendler; Yu-Hui Hu; Florian Wagner; Sabine Thamm; Hans Lehrach; Michal Janitz; Dominique Vanhecke


Book ID
115004886
Publisher
BioMed Central
Year
2008
Tongue
English
Weight
347 KB
Volume
9
Category
Article
ISSN
1471-2164

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## Abstract The analysis of proteinโ€protein interactions is a key focus of proteomics efforts. The yeast twoโ€hybrid system (Y2H) has been the most commonly used method in genomeโ€wide searches for protein interaction partners. However, the throughput of the current yeast twoโ€hybrid array approach is