The yeast two-hybrid technique provides a general approach for cloning cDNAs merely by exploiting the ability of their encoded proteins to bind to a protein of interest. The technique therefore offered a useful access to the analysis of the mechanisms of cell death at the initial stage of their stud
Development of a high-throughput yeast two-hybrid screening system to study protein-protein interactions in plants
✍ Scribed by Y. Fang; D. Macool; Z. Xue; E. Heppard; C. Hainey; S. Tingey; G.-H. Miao
- Publisher
- Springer
- Year
- 2002
- Tongue
- English
- Weight
- 320 KB
- Volume
- 267
- Category
- Article
- ISSN
- 1617-4615
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
## Abstract The analysis of protein‐protein interactions is a key focus of proteomics efforts. The yeast two‐hybrid system (Y2H) has been the most commonly used method in genome‐wide searches for protein interaction partners. However, the throughput of the current yeast two‐hybrid array approach is
Increasing numbers of target protein structures available for computational studies makes the structure-based screening paradigm more attractive for initial hit indentification. We have developed a novel in silico screening methodology incorporating Molecular Mechanics (MM)/implicit solvent methods