The yeast two-hybrid screening technique and its use in the study of protein-protein interactions in apoptosis

✍ Scribed by David Wallach; Mark P Boldin; Andrei V Kovalenko; Nikolai L Malinin; Igor L Mett; Jacques H Camonis

• Publisher: Elsevier Science
• Year: 1998
• Tongue: English
• Weight: 521 KB
• Volume: 10
• Category: Article
• ISSN: 0952-7915
• DOI: 10.1016/s0952-7915(98)80240-9

✦ Synopsis

The yeast two-hybrid technique provides a general approach for cloning cDNAs merely by exploiting the ability of their encoded proteins to bind to a protein of interest. The technique therefore offered a useful access to the analysis of the mechanisms of cell death at the initial stage of their study, when only a few of the proteins involved and very little about their mode of action were known. Conversely, the knowledge of cell death mechanisms gained by this technique provided a useful insight into both the potential and the limitations of this technique.