๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

High-throughput screening: advances in assay technologies

โœ Scribed by G Sitta Sittampalam; Steven D Kahl; William P Janzen

Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
999 KB
Volume
1
Category
Article
ISSN
1367-5931

No coin nor oath required. For personal study only.

โœฆ Synopsis

Both isotopic and nonisotopic assay methodologies are employed in high-throughput screening for drug discovery. Recent advances in cell-based and in vitro biochemical assays will be reviewed, with special emphasis on detection technologies amenable to automated 'mix and read' procedures in high-throughput screening. A major trend is the advent of homogenous assay systems which employ fluorescence resonance energy transfer, fluorescence polarization, and fluorescence correlation spectroscopy. Cell-based assay systems have also become popular in high-throughput screens in which active compounds that directly modulate the disease target are identified. Colorimetric and amperometric methods have also been described recently, but are yet to be adapted widely in high-throughput screens.

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