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High-Throughput Biochemical Kinase Selectivity Assays: Panel Development and Screening Applications

✍ Scribed by Card, Amy ;Caldwell, Chris ;Min, Hyunsuk ;Lokchander, Bina ;Hualin Xi, ;Sciabola, Simone ;Kamath, Ajith V. ;Clugston, Susan L. ;Tschantz, William R. ;Leyu Wang, ;Moshinsky, Deborah J.

Book ID
111643784
Publisher
SAGE Publications
Year
2009
Tongue
English
Weight
413 KB
Volume
14
Category
Article
ISSN
2472-5552

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## Abstract Long‐chain fatty acid esters of 7‐(3,4‐dihydroxybutyloxy)‐2__H__‐1‐benzopyran‐2‐one (**6**) such as octanoate **2a** are shown to be exceptionally sensitive and selective fluorogenic substrates for lipases and esterases. Umbelliferone (**8**) is released upon hydrolysis of the ester fun