In Vitro High-Throughput Screening Assay
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LeΓ³n F. GarcΔ±Μa-MartΔ±Μnez; Graham K. Bilter; Jun Wu; Joe O'Neill; Miguel S. Barb
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Article
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2002
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Elsevier Science
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English
β 459 KB
We developed a 96-well microtiter-plate highthroughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification. Dur