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Development and optimization of high-throughput in vitro protein phosphatase screening assays

✍ Scribed by Tierno, Marni Brisson; Johnston, Paul A; Foster, Caleb; Skoko, John J; Shinde, Sunita N; Shun, Tong Ying; Lazo, John S


Book ID
109946163
Publisher
Nature Publishing Group
Year
2007
Tongue
English
Weight
261 KB
Volume
2
Category
Article
ISSN
1750-2799

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We developed a 96-well microtiter-plate highthroughput screening (HTS) assay for the detection of modulators of transcription. This HTS assay consists of three steps: (1) the in vitro transcription reaction; (2) modification and hybridization of RNA products; and (3) washing and quantification. Dur