High-sensitivity peptide mapping using packed-capillary liquid chromatography and electrospray ionization mass spectrometry
✍ Scribed by J.Fred Banks Jr.
- Book ID
- 107786043
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 379 KB
- Volume
- 743
- Category
- Article
- ISSN
- 1873-3778
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Mass spectrometric techniques for the identification tion of peptides isolated from within the polypeptide of proteins either by amino acid sequencing or by corchain after proteolysis (internal sequencing). Numerrelation of mass spectral data with sequence databases ous protocols for the generation
Capillary electrophoresis/electrospray ionization (CE/ESI) high mass accuracy time-of-flight mass spectrometry was used for the first time to characterize small proteins using peptide mapping. To identify small proteins, the intact proteins were first analyzed to obtain their average molecular weigh
## Abstract The coupling of packed capillary liquid chromatography with electrospray ionization mass spectrometry has yielded an instrument capable of generating molecular weight information from very complex natural protein mixtures. The use of the packed capillary liquid chromatography columns of