Two-dimensional electrophoresis (2-DE) is a highly resolving technique for arraying proteins by isoelectric point and molecular mass. To date, the resolving ability of 2-DE for protein separation is unsurpassed, thus ensuring its use as the fundamental separation method for proteomics. When immobili
High-resolution two-dimensional electrophoresis of myofibrillar proteins with immobilized pH gradients
β Scribed by Jean-Jacques Pernelle; Philippe Chafey; Jean-Luc Lognonne; Pier Giorgio Righetti; Adriana Bianchi Bosisio; Juan P. Wahrmann
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 760 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Protocols for horizontal two-dimensional electrophoresis with immobilized pH gradients in the first dimension were modified for horizontal micro two-dimensional electrophoresis using PhastSystem. Different equilibration conditions of the first-dimensional immobilized pH gradient gel strip prior to s
In this study, metabolically radiolabeled Escherichia coli cell extracts were used to systematically evaluate protein recoveries at each step of two-dimensional (2-D) electrophoresis and using different sample application methods. Sample application using sample cups resulted in better protein recov