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Horizontal two-dimensional electrophoresis with immobilized pH gradients using PhastSystem

✍ Scribed by Dr. Angelika Görg; Wilhelm Postel; Siegfried Günther; Claudia Friedrich

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
293 KB
Volume
9
Category
Article
ISSN
0173-0835

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✦ Synopsis

Protocols for horizontal two-dimensional electrophoresis with immobilized pH gradients in the first dimension were modified for horizontal micro two-dimensional electrophoresis using PhastSystem. Different equilibration conditions of the first-dimensional immobilized pH gradient gel strip prior to second-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis were evaluated. Silver stained two-dimensional patterns were obtained within 3.5 h.

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