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Two-dimensional gel electrophoresis using immobilized pH gradient tube gels

✍ Scribed by Hisashi Hirano; Hiroshi Kawasaki; Hidenori Sassa


Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
463 KB
Volume
21
Category
Article
ISSN
0173-0835

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In this study, metabolically radiolabeled Escherichia coli cell extracts were used to systematically evaluate protein recoveries at each step of two-dimensional (2-D) electrophoresis and using different sample application methods. Sample application using sample cups resulted in better protein recov