✦ LIBER ✦

Improvement of the solubilization of proteins in two-dimensional electrophoresis with immobilized pH gradients

✍ Scribed by Dr. Thierry Rabilloud; Céline Adessi; Anne Giraudel; Joël Lunardi

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 1020 KB
Volume: 18
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.1150180303

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Two-Dimensional Electrophoresis of Membr

Two-Dimensional Electrophoresis of Membrane Proteins Using Immobilized pH Gradients

✍ Mark P. Molloy 📂 Article 📅 2000 🏛 Elsevier Science 🌐 English ⚖ 130 KB

Two-dimensional electrophoresis (2-DE) is a highly resolving technique for arraying proteins by isoelectric point and molecular mass. To date, the resolving ability of 2-DE for protein separation is unsurpassed, thus ensuring its use as the fundamental separation method for proteomics. When immobili

High-resolution two-dimensional electrop

High-resolution two-dimensional electrophoresis of myofibrillar proteins with immobilized pH gradients

✍ Jean-Jacques Pernelle; Philippe Chafey; Jean-Luc Lognonne; Pier Giorgio Righetti 📂 Article 📅 1986 🏛 John Wiley and Sons 🌐 English ⚖ 760 KB

Quantitative evaluation of protein recov

Quantitative evaluation of protein recoveries in two-dimensional electrophoresis with immobilized pH gradients

✍ Xun Zuo; David W. Speicher 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 208 KB 👁 2 views

In this study, metabolically radiolabeled Escherichia coli cell extracts were used to systematically evaluate protein recoveries at each step of two-dimensional (2-D) electrophoresis and using different sample application methods. Sample application using sample cups resulted in better protein recov

The current state of two-dimensional ele

The current state of two-dimensional electrophoresis with immobilized pH gradients

✍ Angelika Görg; Christian Obermaier; Günther Boguth; Alois Harder; Burghardt Sche 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 770 KB

Two-dimensional electrophoresis of prote

Two-dimensional electrophoresis of proteins in an immobilized pH 4–12 gradient

✍ Prof. Dr. Angelika Görg; Günther Boguth; Christian Obermaier; Walter Weiss 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 382 KB 👁 2 views

Improved method for identification of pr

Improved method for identification of proteins using two-dimensional electrophoresis with immobilized pH gradient isoelectric focusing

✍ Dr. Robert B. Mackin; Veronica A. Raker; Joachim Spiess 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 360 KB 👁 1 views

Recent advances in protein sequence analysis now permit the determination of partial N-terminal and internal primary structure from low picomole quantities of protein. The major remaining hurdles to sequence analysis of small amounts of protein are the identification, isolation, and handling of micr