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High-pressured liquid chromatography of carotenoids

✍ Scribed by Anne Fiksdahl; Julius T. Mortensen; Synnøve Liaaen-Jensen

Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
443 KB
Volume
157
Category
Article
ISSN
1873-3778

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✦ Synopsis

The potential of high-pressure liquid chromatography @PLC) for separation of carotenoids on silica has been studied with selected model carotenoids (carotenes, diols, cis-trans isomers and diastereoisomers) in comparison with conventional thinlayer chromatography and-circular paper techniques. Fast and efficient separation on the analytical scale was obtained, including that of epimers not previously separated_ The advantage of HPLC coupled with a LJV-VIS scanning spectrophotometer for the analysis of cis-frans isomerization mixtures of carotenoids is pointed out.

' Mobile phase: acetonehexane (20:80).

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