The size-dependent separation of viral and subviral particles in the range lo'-IO7 daltons was undertaken by high-performance liquid chromatography. A combination of Ultrahydrogel 2000 and 1000 size-exclusion columns, equilibrated and developed with Tris buffer (pH 7.4) was used to fractionate extr
High-performance size exclusion chromatography as a rapid method for the separation of steroid hormone receptors
β Scribed by Ronald D. Wiehle; Glen E. Hofmann; Axel Fuchs; James L. Wittliff
- Book ID
- 108449712
- Publisher
- Elsevier Science
- Year
- 1984
- Weight
- 997 KB
- Volume
- 307
- Category
- Article
- ISSN
- 0378-4347
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Thin-layer chromatogram (6 x 6 cm) of the model mixture: (1) inorganic phosphate, (2) choline, (3) choline phosphate, (4) glycerophosphorylcholine, (5) glycerophosphate, (6) betaine. Solvent systems: First direction (vertical), methanol/water/conc. NH4 OH/chlorotorm/acetone (5 : 2 : 1 : 2 : 2); Seco