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High-performance size-exclusion chromatography: A buffer for the reliable determination of molecular weights of proteins

✍ Scribed by Franz Hefti

Publisher: Elsevier Science
Year: 1982
Tongue: English
Weight: 254 KB
Volume: 121
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(82)90496-1

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✦ Synopsis

A high-performance size-exclusion chromatographic system is described that minimizes the ionic and hydrophobic interactions of proteins with the stationary phase. The system can be used to determine reliably the molecular weight of proteins between M, 10,000 and 70,000. A further application would be to separate proteins on a preparative (milligram) scale. Conditions critical for optimal resolution are discussed.

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