## Abstract A video fluorometer is used as a detector for HPLC to characterize highly complex samples. Data selection and evaluation techniques are discussed so as to speed the spectral identification process. Several wellβknown polynuclear aromatic hydrocarbons are found in the samples.
High performance liquid chromatography/video fluorometry. Part I: Instrumentation
β Scribed by Fogarty, M. P. ;Shelly, D. C. ;Warner, I. M.
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 759 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0935-6304
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β¦ Synopsis
Abstract
A rapid scanning, twoβdimensional fluorometer has been used to obtain EmissionβExcitation Matrices (EEMs) of the effluent of an HPLC column. These spectra, obtained βon the flyβ, provide qualitative as well as quantitative information about unknown samples. An inβdepth discussion of the instrumentation developed and an account of data processing alternatives are presented.
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