Gel filtration chromatography has been used to analyze the oligomerization of human erythrocyte spectrin. By applying an exponentially modified Gaussian function we have been able to resolve overlapping elution peaks. From these peaks it was possible to calculate the equilibrium composition of each
Analysis of sorbitan ester surfactants. Part I: High performance liquid chromatography
β Scribed by Wang, Zhendi ;Fingas, Merv
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 441 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0935-6304
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β¦ Synopsis
Abstract
Sorbitan ester surfactants are very complicated mixtures. An improved and simple reversed phase high performance liquid chroma tographic (HPLC) method employing a C~18~ column has been developed for the rapid separation of sorbitan ester surfactants and the quantitative determination of the distribution of sorbitan monoβ, diβ, triβ, and tetraester fractions. The sorbitan ester distribution of the six Span and Arlacel surfactants studied was calculated using relative response factors obtained from analysis of pure glycerides of fatty acids. The effects of mobile phase composition on the accuracy and reliability of the distribution values calculated for the sorbitan esters were investigated.
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