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High-Performance Liquid Chromatography Analysis of Spectrin Oligomerization

✍ Scribed by Lars Löfvenberg; Lars Backman

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
81 KB
Volume
292
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Gel filtration chromatography has been used to analyze the oligomerization of human erythrocyte spectrin. By applying an exponentially modified Gaussian function we have been able to resolve overlapping elution peaks. From these peaks it was possible to calculate the equilibrium composition of each spectrin concentration and thus also the dissociation constants describing the oligomeric process. The determined dissociation constants for tetramer formation (1.3 M) and for hexamer formation (24 M) agree well with other measurements.

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