High-performance liquid chromatography-fluorescent assay of catechol-O-methyltransferase activity in rat brain
β Scribed by Mayumi Masuda; Makoto Tsunoda; Kazuhiro Imai
- Book ID
- 105888324
- Publisher
- Springer
- Year
- 2003
- Tongue
- English
- Weight
- 182 KB
- Volume
- 376
- Category
- Article
- ISSN
- 1618-2650
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π SIMILAR VOLUMES
The soluble form of catechol-0-methyltransferase (EC 2.1.1.6.) from rat liver was purified to homogeneity by high-performance anion-exchange chromatography and high-performance gel-filtration chromatography. The specific activity of the final pool was 270 U/mg protein. The purification was 1180-fold
A sensitive assay for catechol-O-methyltransferase (COMT) activity by high-performance liquid chromatography with on-line radiochemical detection was described. The method was based on the measurement of 3H-labeled 3-O- and 4-O-methylated products of the substrate, 3,4-dihydroxybenzoic acid, using S